The Onco-Genomics Shared Resource (OGSR) is a state-of-the-art shared resource located in the Papanicolaou Cancer Research Building (PAP) on the University of Miami, Miller School of Medicine campus. We provide Sylvester investigators access to a wide range of services and expertize for the study of the genomics and epigenetics of cancer. Principle service areas are: 1) Extensive support for next generation sequencing (NGS), 2) Single-cell & spatial genomics 3) Gene expression assays and molecular quantitation, 4) Sample extraction quantitation and QC.
The OGSR is a component of the NCI Sylvester Cancer Center Support Grant (P30 CA240139).
To request services from the OGSR click on the Initiate Request tab on the upper right.
|
Instrument |
Description |
|
Illumina NovaSeq X Plus |
Production scale, short read NGS sequencer |
|
PE Zephyr G3 NGS |
Automated liquid handler for NGS library prep |
|
Hamilton Vantage 2.0 |
Automated liquid handler for NGS library prep |
|
UVP UV3 HEPA cabs (two) |
HEPA filtered PCR workstations with UV air irradiation |
|
BSL2 safety cabinet (two) |
HEPA filtered circulating air safety cabinets |
|
10x Genomics Chromium X |
Single cell controller for genomics assays |
|
Mission Bio Tapestri |
Single cell controller for genomics assays |
|
Nexcelom K2 |
Imaging cell counter with 2 channel fluorescence |
|
Bruker Spatial Biology GeoMx |
Digital spatial profiler (DSP) for ROI-driven multiomics |
|
Bruker Spatial Biology CosMx |
Spatial Molecualr Imager (SMI) for in situ transcriptomics |
|
Agilent 5200 |
12 capillary DNA/RNA fragment analyzer |
|
Agilent Femto Pulse |
12 capillary DNA/RNA pulse field HMW fragment analyzer |
|
Thermo Qubit 3.0 |
Single tube fluorimeter for dye-based DNA/RNA quantification |
|
Bio-Rad PTC Tempo (four) |
Thermal cyclers with deep-well blocks |
|
Bio-Rad C1000 (two) |
Thermal cyclers with deep-well and standard blocks |
|
Bio-Rad CFX96 |
Multichannel real-time PCR machine |
|
Qiagen QIAcuity ONE |
5 channel, nano-well digital PCR machine |
|
Private Server |
28Tb Central Computing Services |
|
Sylvester Data Portal Storage |
Cloud storage with UI for investigators |
| Hours | Location |
|
Mon-Fri 9:00AM - 5:00PM |
Onco-Genomics Shared Resource (OGSR) PAP 414 |
Telephone: 305 243 7927
Email: OGSR@miami.edu