Onco-Genomics Shared Resource (OGSR)

Overview

 

The Onco-Genomics Shared Resource (OGSR) is a state-of-the-art shared resource located in the Papanicolaou Cancer Research Building (PAP) on the University of Miami, Miller School of Medicine campus. We provide Sylvester investigators access to a wide range of services and expertize for the study of the genomics and epigenetics of cancer. Principle service areas are: 1) Extensive support for next generation sequencing (NGS), 2) Single-cell & spatial genomics 3) Gene expression assays and molecular quantitation, 4) Sample extraction quantitation and QC.

The OGSR is a component of the NCI Sylvester Cancer Center Support Grant (P30 CA240139).

 

To request services from the OGSR click on the Initiate Request tab on the upper right.

 

Instrument

Description

Illumina NovaSeq X Plus

Production scale, short read NGS sequencer

PE Zephyr G3 NGS

Automated liquid handler for NGS library prep

Hamilton Vantage 2.0

Automated liquid handler for NGS library prep

UVP UV3 HEPA cabs (two)

HEPA filtered PCR workstations with UV air irradiation

BSL2 safety cabinet (two)

HEPA filtered circulating air safety cabinets

10x Genomics Chromium X

Single cell controller for genomics assays

Mission Bio Tapestri

Single cell controller for genomics assays

Nexcelom K2

Imaging cell counter with 2 channel fluorescence

Bruker Spatial Biology GeoMx

Digital spatial profiler (DSP) for ROI-driven multiomics

Bruker Spatial Biology CosMx

Spatial Molecualr Imager (SMI) for in situ transcriptomics

Agilent 5200

12 capillary DNA/RNA fragment analyzer

Agilent Femto Pulse

12 capillary DNA/RNA pulse field HMW fragment analyzer

Thermo Qubit 3.0

Single tube fluorimeter for dye-based DNA/RNA quantification

Bio-Rad PTC Tempo (four)

Thermal cyclers with deep-well blocks

Bio-Rad C1000 (two)

Thermal cyclers with deep-well and standard blocks

Bio-Rad CFX96

Multichannel real-time PCR machine

Qiagen QIAcuity ONE

5 channel, nano-well digital PCR machine

Private Server

28Tb Central Computing Services

Sylvester Data Portal Storage

Cloud storage with UI for investigators

 

Location and hours of operation

Hours Location 

Mon-Fri 9:00AM - 5:00PM 

Onco-Genomics Shared Resource (OGSR)

PAP 414
1550 N.W. 10th Avenue
Miami, FL 33136

Links and Resources

Telephone: 305 243 7927 

Email: OGSR@miami.edu

sylvester.org/ogsr

Map